Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay.

نویسندگان

  • Tiffany P Gustafson
  • Greg A Metzel
  • Andrei G Kutateladze
چکیده

Amplified fluorescence quenching methodology based on massive autocatalytic photo-unmasking of a dual function sensitizer-quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin-avidin pair, achieving attomolar detection.

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عنوان ژورنال:
  • Organic & biomolecular chemistry

دوره 9 13  شماره 

صفحات  -

تاریخ انتشار 2011